Self-Amplifying RNA (saRNA) Synthesis Service
Advance Your Research with Echo Bio's Cutting-Edge saRNA Solutions
Talk to Our ExpertsTransform Your RNA Research with saRNA Innovation
Echo Bio is a leader in RNA synthesis, offering end-to-end solutions for self-amplifying RNA (saRNA) production. Our proprietary technologies leverage viral replicase elements to enable self-replication, delivering sustained, high-level protein expression from minimal doses. Ideal for next-generation vaccines, CAR-T therapies, and protein replacement, our saRNA outperforms traditional mRNA with lower dosing, prolonged activity, and cost-effective production.
Why Choose Echo Bio for saRNA Synthesis?
- One-Stop Solution: Comprehensive services from sequence optimization to lipid nanoparticle (LNP) formulation.
- Proven Quality: Over 70 rigorous quality control assays ensure high purity and performance.
- High Expression: Up to 10x higher and longer-lasting protein expression compared to linear mRNA.
- Safe & Scalable: Non-integrating, high-tolerability saRNA with production from micrograms to grams.
Our saRNA Service Capabilities
We provide a customizable pipeline to deliver high-quality saRNA for diverse applications:
- Sequence Optimization: Proprietary codon and UTR tuning for maximal replicase and transgene expression.
- Gene Synthesis & Cloning: High-fidelity synthesis (100 bp–6 kb) with stable poly(A) tails.
- In Vitro Transcription (IVT): Advanced IVT with modified nucleotides to enhance yield and reduce immunogenicity.
- Capping Excellence: >95% capping efficiency, optimized for alphavirus replication.
- LNP Formulation: Optional encapsulation for targeted in vivo delivery (e.g., spleen, lymph nodes).
Comprehensive Quality Control
Our saRNA products undergo over 70 quality control assays to ensure unmatched quality and performance:
| Attribute | QC Assay | Specification |
|---|---|---|
| Identity | Sanger Sequencing | Matches Target Sequence |
| mRNA Length | Capillary Gel Electrophoresis | Target ±30% |
| Concentration | UV Spectrophotometry | Target ±5% |
| Capping Efficiency | LC-MS | ≥95% |
| Purity (A260/280) | UV Spectrophotometry | 1.8-2.2 |
| Endotoxin | Kinetic Chromogenic Assay | <5 EU/mg |
| Residual dsRNA | Dot Blot Assay | Undetectable |
| Poly(A) Length | Enzyme Digestion & CE | Target ±10 nt |
Each batch includes a detailed Analytical Report or Certificate of Analysis.
Explore Our Analytical CapabilitiesExplore Our RNA Catalog Products
In addition to custom saRNA, we offer:
- Linear mRNA: High-purity mRNA for vaccines and gene expression studies.
- Circular RNA (circRNA): Stable, long-lasting RNA for advanced therapeutics.
- Self-Amplifying RNA (saRNA): Ready-to-use EGFP and FLuc controls for optimization.
- LNP Formulations: Optimized delivery systems for efficient RNA administration.