Specification
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Expression Host | E.coli |
| Tag Info | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P95434 |
| Gene Names | pscF |
| Alternative Names | Pseudomonas secretion protein F |
| Expression Region | Full Length of Mature Protein(2-85aa ) |
| Molecular Weight | 16.6 kDa |
| Protein Sequence | AQIFNPNPGNTLDTVANALKEQANAANKDVNDAIKALQGTDNADNPALLAELQHKINKWSVIYNINSTVTRALRDLMQGILQKI |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Major component of the type III secretion needle structure. Required for cytotoxicity on macrophages. |
| Involvement in Disease | |
| Subcellular Location | Secreted |
| Protein Families | MxiH/PrgI/YscF family |
| Tissue Specificity | pscF |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |