Specification
| Organism | Homo sapiens (Human) |
| Expression Host | E.Coli |
| Tag Info | N-terminal 6xHis-tagged |
| Purity | >95% as determined by SDS-PAGE and HPLC. |
| Uniprot ID | P09486 |
| Uniprot Entry Name | SPRC_HUMAN |
| Gene Names | SPARC,ON |
| Alternative Names | Basement-membrane protein 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, |
| Expression Region | Full Length of Mature Protein (18-303aa) |
| Molecular Weight | 36.1 kDa |
| Endotoxin | Less than 1.0 EU/µg as determined by LAL method. |
| Sequence | MSYYHHHHHH DYDIPTTENL YFQGAMGSA+P QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI |
| Product Form | Lyophilized powder (Lyophilized from a 0.2 µm filtered PBS, pH 7.4) |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Involvement in disease | Osteogenesis imperfecta 17 (OI17) |
| Subcellular Location | Secreted, extracellular space, extracellular matrix, basement membrane |
| Protein Families | SPARC family |
| Tissue Specificity | |
| Pathway |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |