Specification
| Description | Recombinant protein from the full-length sequence of Homo sapiens replication protein A4 (RPA4) (NM_013347). |
| Organism | Homo sapiens (Human) |
| Expression Host | Human Cells |
| Tag Info | His or DYKDDDDK. Please contact us if you need further information or require specific designed tag. |
| Purity | Greater than 90% by SDS-PAGE gel |
| Uniprot ID | Q13156 |
| Entry Name | RFA4_HUMAN |
| Gene Names | RPA4 |
| Alternative Gene Names | |
| Alternative Protein Names | Replication protein A 30 kDa subunit (RP-A p30) (Replication factor A protein 4) (RF-A protein 4) |
| Application | Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only! |
| Buffer | Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (<1EU /µg) |
| Length | 261 |
| Molecular Weight(Da) | 28868 |
| Protein Sequence | (The sequence of expressed protein may have some variation from the sequence shown below. Please contact us for the exact sequence.) MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIHECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSAD |
Background
| Function | FUNCTION: As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. {ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:19942684, ECO:0000269|PubMed:19996105, ECO:0000269|PubMed:20545304}. |
| Pathway | |
| Protein Families | Replication factor A protein 2 family |
| Tissue Specificity | Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels. {ECO:0000269|PubMed:19996105}. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |