Specification
| Description | Recombinant protein from the full-length sequence of Homo sapiens proteasomal ATPase associated factor 1 (PAAF1), transcript variant 4 (NM_025155). |
| Organism | Homo sapiens (Human) |
| Expression Host | Human Cells |
| Tag Info | His or DYKDDDDK. Please contact us if you need further information or require specific designed tag. |
| Purity | Greater than 90% by SDS-PAGE gel |
| Uniprot ID | Q9BRP4 |
| Entry Name | PAAF1_HUMAN |
| Gene Names | PAAF1 WDR71 |
| Alternative Gene Names | WDR71 |
| Alternative Protein Names | Proteasomal ATPase-associated factor 1 (Protein G-16) (WD repeat-containing protein 71) |
| Application | Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only! |
| Buffer | Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (<1EU /µg) |
| Length | 392 |
| Molecular Weight(Da) | 42190 |
| Protein Sequence | (The sequence of expressed protein may have some variation from the sequence shown below. Please contact us for the exact sequence.) MAAPLRIQSDWAQALRKDEGEAWLSCHPPGKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL |
Background
| Function | FUNCTION: Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation. {ECO:0000269|PubMed:15831487, ECO:0000269|PubMed:17289585, ECO:0000269|PubMed:22316138}. |
| Pathway | |
| Protein Families | WD repeat PAAF1/RPN14 family |
| Tissue Specificity | Ubiquitously expressed, with highest levels in kidney, brain and testis. {ECO:0000269|PubMed:15831487}. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |