Specification
| Organism | Homo sapiens (Human) |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | Q03001 |
| Gene Names | DST |
| Alternative Names | 230KDA bullous pemphigoid antigen 230/240KDA bullous pemphigoid antigen Bullous pemphigoid antigen 1 Short name:BPA Short name:Bullous pemphigoid antigen |
| Expression Region | Partial of Isoform 3(1-195aa ) |
| Molecular Weight | 39.1 kDa |
| Protein Sequence | MHSSSYSYRSSDSVFSNTTSTRTSLDSNENLLLVHCGPTLINSCISFGSESFDGHRLEMLQQIANRVQRDSVICEDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSG |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Cytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Isoform 3: plays a structural role in the assembly of hemidesmosomes of epithelial cells; anchors keratin-containing intermediate filaments to the inner plaque of hemidesmosomes. Required for the regulation of keratinocyte polarity and motility; mediates integrin ITGB4 regulation of RAC1 activity. Isoform 6: required for bundling actin filaments around the nucleus.By similarity3 Publications Isoform 7: regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport. |
| Involvement in Disease | Neuropathy, hereditary sensory and autonomic, 6 (HSAN6); Epidermolysis bullosa simplex, autosomal recessive 2 (EBSB2) |
| Subcellular Location | Cytoplasm, cytoskeleton, Cell projection, axon, Note=Associates with intermediate filaments, actin and microtubule cytoskeletons, Localizes to actin stress fibers and to actin-rich ruffling at the cortex of cells (By similarity), Associated at the growing distal tip of microtubules, SUBCELLULAR LOCATION: Isoform 1: Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line, Cytoplasm, myofibril, sarcomere, H zone, Note=Localizes to microtubules and actin microfilaments throughout the cytoplasm and at focal contact attachments at the plasma membrane, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, cytoskeleton, Note=Colocalizes both cortical and cytoplasmic actin filaments, SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytoskeleton, Cell junction, hemidesmosome, Note=Localizes to actin and intermediate filaments cytoskeletons (By similarity), Colocalizes with the epidermal KRT5-KRT14 intermediate filaments network of keratins, Colocalizes with ITGB4 at the leading edge of migrating keratinocytes, SUBCELLULAR LOCATION: Isoform 6: Nucleus, Nucleus envelope, Membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein, Cytoplasm, cytoskeleton, Note=Localizes to actin and intermediate filaments cytoskeletons, Localizes to central actin stress fibers around the nucleus and is excluded form focal contact sites in myoblast cells, Translocates to the nucleus (By similarity), Associates with actin cytoskeleton in sensory neurons, SUBCELLULAR LOCATION: Isoform 7: Cytoplasm, cytoskeleton, Cell projection, axon, Membrane |
| Protein Families | |
| Tissue Specificity | DST |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |