Specification
| Organism | Homo sapiens (Human) |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P00374 |
| Gene Names | DHFR |
| Alternative Names | DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3 |
| Expression Region | Full Length(1-187aa ) |
| Molecular Weight | 25.5 kDa |
| Protein Sequence | MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Involvement in Disease | Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD) |
| Subcellular Location | Mitochondrion, Cytoplasm |
| Protein Families | Dihydrofolate reductase family |
| Tissue Specificity | DHFR |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |