Specification
| Organism | Escherichia coli |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P33789 |
| Gene Names | traY |
| Alternative Names | traY; Relaxosome protein TraY; Fragment |
| Expression Region | Full Length(1-20aa ) |
| Molecular Weight | 18.2 kDa |
| Protein Sequence | MKEPKSNIRKLIDIGGLIGR |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI. Also positively regulates tra gene expression |
| Involvement in Disease | |
| Subcellular Location | Cytoplasm |
| Protein Families | TraY family |
| Tissue Specificity | traY |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |