Specification
| Organism | Escherichia coli (strain K12) |
| Expression Host | E.coli |
| Tag Info | N-terminal GST-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P0AG55 |
| Gene Names | rplF |
| Alternative Names | rplF; b3305; JW3267; 50S ribosomal protein L6; Large ribosomal subunit protein uL6 |
| Expression Region | Partial(2-175aa ) |
| Molecular Weight | 45.5 kDa |
| Protein Sequence | SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.Gentamicin-resistant mutations in this protein affect translation fidelity. |
| Involvement in Disease | |
| Subcellular Location | |
| Protein Families | Universal ribosomal protein uL6 family |
| Tissue Specificity | rplF |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |