Specification
| Organism | Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps) |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P0C1Z0 |
| Gene Names | Fas-2 |
| Alternative Names | Short name:Fas-2 Short name:Fas2 Alternative name(s): Acetylcholinesterase toxin F-VII Fasciculin-II Short name:FAS-II Toxin TA1 |
| Expression Region | Full Length(1-61aa ) |
| Molecular Weight | 22.8 kDa |
| Protein Sequence | TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1:1 stoichiometry the mammalian and electric fish AChE at picomolar concentrations. It is highly specific for the peripheral site of AChE and blocks the entry of acetylcholine into the active site of the enzyme (through the Met-33 residue), thereby preventing its breakdown. It has been called fasciculin since after injection into mice it causes severe, generalized and long-lasting (5-7 hours) fasciculations. |
| Involvement in Disease | |
| Subcellular Location | Secreted |
| Protein Families | Snake three-finger toxin family, Short-chain subfamily, Acn-esterase inhibitor sub-subfamily |
| Tissue Specificity | Fas-2 |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |