Specification
| Organism | Chlamydomonas reinhardtii (Chlamydomonas smithii) |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P31178 |
| Gene Names | N/A |
| Alternative Names | Gamete lytic enzyme |
| Expression Region | Full Length of Mature Protein(184-638aa ) |
| Molecular Weight | 65.6 kDa |
| Protein Sequence | EIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. |
| Involvement in Disease | |
| Subcellular Location | Periplasm, Secreted, cell wall |
| Protein Families | Peptidase M11 family |
| Tissue Specificity | N/A |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |