Specification
| Organism | Avian infectious bursal disease virus (strain Cu-1) (IBDV) (Gumboro disease virus) |
| Expression Host | E.coli |
| Tag Info | N-terminal 6xHis-tagged |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P15480 |
| Gene Names | N/A |
| Alternative Names | Structural polyprotein; PP) [Cleaved into: Precursor of VP2; Pre-VP2); Capsid protein VP2; Structural peptide 1; p1; pep46); Structural peptide 2; p2; pep7a); Structural peptide 3; p3; pep7b); Structural peptide 4; p4; pep11); Protease VP4; EC 3.4.21.-; Non-structural protein VP4; NS); Capsid protein VP3] |
| Expression Region | Partial(723-1012aa ) |
| Molecular Weight | 36.8 kDa |
| Protein Sequence | RFPHNPRDWDRLPYLNLPYLPPNAGRQYHLAMAASEFKETPELESAVRAMEAAANVDPLFQSALSVFMWLEENGIVTDMANFALSDPNAHRMRNFLANAPQAGSKSQRAKYGTAGYGVEARGPTPEEAQREKDTRISKKMETMGIYFATPEWVALNGHRGPSPGQLKYWQNTREIPDPNEDYLDYVHAEKSRLASEEQILRAATSIYGAPGQAEPPQAFIDEVAKVYEINHGRGPNQEQMKDLLLTAMEMKHRNPRRALPKPKPKPNAPTQRPPGRLGRWIRTVSDEDLE |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Capsid protein VP2 self assbles to form an icosahedral capsid with a T=13 symmetry, about 70 nm in diameter, and consisting of 260 VP2 trimers. The capsid encapsulates the genomic dsRNA. VP2 is also involved in attachment and entry into the host cell by interacting with host ITGA4/ITGB1 .The precursor of VP2 plays an important role in capsid assbly. First, pre-VP2 and VP2 oligomers assble to form a procapsid. Then, the pre-VP2 intermediates may be processed into VP2 proteins by proteolytic cleavage mediated by VP4 to obtain the mature virion. The final capsid is composed of pentamers and hexamers but VP2 has a natural tendency to assble into all-pentameric structures. Therefore pre-VP2 may be required to allow formation of the hexameric structures .Protease VP4 is a serine protease that cleaves the polyprotein into its final products. Pre-VP2 is first partially cleaved, and may be completely processed by VP4 upon capsid maturation.. |
| Involvement in Disease | |
| Subcellular Location | Capsid protein VP2: Virion, Host cytoplasm, SUBCELLULAR LOCATION: Capsid protein VP3: Virion, Host cytoplasm, SUBCELLULAR LOCATION: Structural peptide 1: Virion, Host cytoplasm, SUBCELLULAR LOCATION: Structural peptide 2: Virion, Host cytoplasm, SUBCELLULAR LOCATION: Structural peptide 3: Virion, Host cytoplasm, SUBCELLULAR LOCATION: Structural peptide 4: Virion, Host cytoplasm |
| Protein Families | |
| Tissue Specificity | N/A |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |