Specification
| Organism | Apis mellifera (Honeybee) |
| Expression Host | E.coli |
| Tag Info | Tag-Free |
| Purity | Greater than 85% by SDS-PAGE |
| Uniprot ID | P01501 |
| Gene Names | MELT |
| Alternative Names | MEL (MLT) (Allergen Api m 3) (Allergen Api m III) (Allergen: Api m 4) |
| Expression Region | Full Length of Mature Protein(44-69aa ) |
| Molecular Weight | 2.8 kDa |
| Protein Sequence | GIGAVLKVLTTGLPALISWIKRKRQQ |
| Form | Liquid or Lyophilization |
| Buffer | The default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol if the delivery form is liquid. The lyophilization buffer is Tris/PBS-based buffer, 6% Trehalose, pH 8.0 if the delivery form is lyophilized powder. Please contact us if you have any special requirment. |
| Reconstitution | Please reconstitute protein in deionized sterile water and we recommend that briefly centrifuge thevial prior to opening the vial .We recommend aliquot for long-term storage at -20℃/-80℃. |
Background
| Relevance | Melittin: Main toxin of bee venom with strong hemolytic activity and antimicrobial activity . It has enhancing effects on bee venom phospholipase A2 activity . This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis . It acts as a voltage-gated pore with higher selectivity for anions over cations . The ion conductance has been shown to be voltage-dependent . Self-association of melittin in membranes is promoted by high ionic strength, but not by the presence of negatively charged lipids . In vivo, intradermal injection into healthy human volunteers produce sharp pain sensation and an inflammatory response . It produces pain by activating primary nociceptor cells directly and indirectly due to its ability to activate plasma membrane phospholipase A2 and its pore-forming activity . |
| Involvement in Disease | |
| Subcellular Location | |
| Protein Families | |
| Tissue Specificity | MELT |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |