Specification
| Target | GFP |
| Conjugate | Agarose Beads |
| Application | IP, CHIP, MS, PURIFICATION |
| Conjugate | Agarose Beads |
| Host | Alpaca pacous |
| Isotype | VHH domain of alpaca IgG2b/2c |
| Reactivity | Binds to GFP, mEGFP, superfolder GFP, and most common CFP and YFP variants, but does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP, or their derivatives |
| Purificatin | |
| Concentration | |
| Buffer | 50 % slurry in PBS containing 20 % Ethanol |
| Storage | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze. |
| Advantages | · High affinity · Lot to lot consistency · Short Incubation |
Background
| Background | VHH or Nanobody is the next generation monoclonal antibody, whichn is a Single domain antibody (sdAb) reserving only one single antigen recognizing domain from camelid heavy chain antibody. With size of only ~ 15 kDa, Nanobody dispay numerous advantages over conventional antibody and it's derivatives, such as higher tissue penetration capability, more accessible to hidden epitopes, higher stability(thermal and pH resistant), solubility (aggregation avoidance) and binding affinity. Fluorescent protein GFP frequently used as reporter and fusion tag. Anti-GFP Nanobody Beads are magnetic agarose beads covalently coupled with VHH antibodies/ nanobodies acquiring high specificity and affinity for GFP. These nanobeads can efficiently capture and separate GFP and GFP tagged proteins from cell crudes or other samples. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |