Specification
| Description | Recombinant protein from the full-length sequence of Homo sapiens phosphoethanolamine/phosphocholine phosphatase 1 (PHOSPHO1), transcript variant 2 (NM_178500). |
| Organism | Homo sapiens (Human) |
| Expression Host | Human Cells |
| Tag Info | His or DYKDDDDK. Please contact us if you need further information or require specific designed tag. |
| Purity | Greater than 90% by SDS-PAGE gel |
| Uniprot ID | Q8TCT1 |
| Entry Name | PHOP1_HUMAN |
| Gene Names | PHOSPHO1 |
| Alternative Gene Names | |
| Alternative Protein Names | Phosphoethanolamine/phosphocholine phosphatase (EC 3.1.3.75) |
| Application | Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only! |
| Buffer | Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (<1EU /µg) |
| Length | 267 |
| Molecular Weight(Da) | 29713 |
| Protein Sequence | (The sequence of expressed protein may have some variation from the sequence shown below. Please contact us for the exact sequence.) MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC |
Background
| Function | FUNCTION: Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed:15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity). {ECO:0000250|UniProtKB:Q8R2H9, ECO:0000269|PubMed:15175005}. |
| Pathway | |
| Protein Families | HAD-like hydrolase superfamily, PHOSPHO family |
| Tissue Specificity | Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. {ECO:0000269|PubMed:15050893}. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |